Basespace fastq download some files fail some ok

In general, for a given sample, the percentage of demultiplexed reads should be similar across all tiles. The tile summary information can be used to identify tile-specific demultiplexing issues. Some common causes for poor demultiplexing that these lists can reveal are: Index sequences entered in the wrong orientation in the sample sheet. Sample mix ups between lanes. Poor Index Read sequencing quality. Ns in the sequences represent positions where the base calling software was unable to make a base call.

Poly-G sequences are typical for PhiX reads, which are not indexed. Contact Us Technical Support techsupport illumina. Share Desktop. Other Support Safety Data Sheets. Product Lot Tracker. Take Back and Recycle Program. The value is the decimal representation of a bit-wise encoding scheme. In that scheme bit 0 has a decimal value of 1, bit 1 a value of 2, bit 2 a value of 4, and so on.

Toggle navigation. Introduction What is it? FASTQ files can be used as sequence input for alignment and other secondary analysis software. Detailed Description Naming FASTQ files are named with the sample name and the sample number, which is a numeric assignment based on the order that the sample is listed in the sample sheet.

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